Correlation between fms-Like Tyrosine Kinase-1 (sFlt-1) Cell-Free Messenger RNA Expression and fms-Like Tyrosine Kinase-1 (sFlt-1) Protein Level in Severe Preeclampsia and Normal Pregnancy
Jeffry Iman Gurnadi
Johanes Cornelius Mose
Prima Nanda Fauziah
Akhmad Yogi Pramatirta
International Journal of Integrated Health Sciences. 2015;3(2):66-71
Objective: To analyze sFlt-1 and sFlt-1 mRNA levels in severe preeclampsia and normal pregnancy, and the correlation between both in occurrence of severe preeclampsia.
Methods: This is a cross-sectional analytic observational study involving 18 subjects with severe preeclampsia and 19 subjects with normal pregnancy as controls who met inclusion and exclusion criteria. Levels of sFlt-1 and sFlt-1 mRNA were measured with ELISA and RT PCR. Statistical analysis was performed with Chi square test, Fisher’s exact test, T-test, Mann-Whitney test, and Spearman’s rank correlation test.
Results: This study showed no significant difference (p>0.05) in the characteristics of maternal age, gestational age, and parity in both study groups. Mean level of sFlt-1 mRNA in severe preeclampsia was higher (6.3404 pg/mL) compared to its level in normal pregnancy (5.9701 pg/mL). There was an insignificant (p>0.05) positive correlation between sFlt-1 mRNA and sFlt-1 levels in normal pregnancy, and an insignificant (p>0.05) negative
correlation between both levels in severe preeclampsia.
Conclusions: sFlt-1 mRNA levels in severe preeclampsia are higher than its level in normal pregnancy. There is no correlation between sFlt-1 mRNA level and sFLt-1 protein level in severe preeclampsia. There is an insignificant
positive correlation between sFlt-1 mRNA and sFlt-1 levels in normal pregnancy, and an insignificant negative correlation between both levels in severe preeclampsia.
Keywords: Normal pregnancy, severe preeclampsia, sFlt-1 mRNA