TGFB1 and TGFBR2 gene polymorphisms and pathophysiology of severe preeclampsia

Anita Deborah Anwar
Firman Fuad Wirakusumah
Sofie Rifayani Krisnadi
Tono Djuwantono
Yelliantty
Leri Septiani

American Journal of Research Communication

Abstract
Transforming growth factor β1 (TGFB1) and transforming growth factor β type 2 receptor (TGFBR2) are key components of TGF-β signaling and play an important role in vascular remodeling in placentation. Several polymorphisms have been identified in TGFB1 and TGFBR2 genes, including those associated with the regulation of TGF-β1 levels and TGF binding to its receptor. The decrease of TGF-β1 levels and the disruption of TGF TGF-β1 signaling can cause abnormal placentation, hypoxia, and increased soluble endoglin (sEng), which can lead to preeclampsia.This study aims to determine the role of gene polymorphisms C-509T TGFB1 and C1167T TGFBR2and their relation to TGF-β1 and sEng levels in cases of severe preeclampsia. Genotyping performed using restriction fragment length polymorphism (RFLP) and a direct sequencing method was used to study 26 cases of preeclampsia against 26 control matched normal pregnancies. The results showed that the TGF-β1 levels in severe preeclampsia were significantly lower compared to normal pregnancies, whereas the levels of sEng in severe preeclampsia were significantly higher compared to normal pregnancies. In addition, the genotypes -509CT/TT TGFB1 and 1167CT/TT TGFBR2 appeared in both groups. This analysis highlights the absence of a significant association between the genotype -509C/T TGFB1 and TGF-β1 levels in both groups.

The results did not indicate any significant associations between the genotypes – 509C/T TGFB1 and 1167C/T TGFBR2 and the levels of sEng in both groups. Based on these results, it can be concluded that the levels of TGF-β1 and sEng are associated with severe preeclampsia but that the gene polymorphisms C-509T TGFB1 and C1167T TGFBR2 are not directly related to severe preeclampsia.

Key Words: Severe preeclampsia, transforming growth factor β1, transforming growth
factor β type 2 receptor, single nucleotide polymorphism, soluble endoglin
{Citation: Anita Deborah Anwar, Firman Fuad Wirakusumah, Sofie Rifayani Krisnadi, Tono
Djuwantono, Yelliantty, and Leri Septiani. TGFB1 and TGFBR2 gene polymorphisms and
pathophysiology of severe preeclampsia. American Journal of Research Communication,
2015, 3(6): 28-44} www.usa-journals.com, ISSN: 2325-4076.

Link